It contains five anatomic layers, which are the epithelium, Bowman’s layer, the stroma, Descemet’s membrane and the endothelium. The vertebrate cornea is a dome-shaped transparent outer surface of the eye.
Taken together, our data suggested that Shp2-mediated MAPK pathway regulated ΔNp63, which in turn positively regulated Ngf in epithelium to warrant corneal innervation and epithelial homeostasis More interesting, co-transfection experiments revealed that Shp2 tightly monitored ΔNp63 protein level in HEK293 and TKE2 cells. Furthermore, ∆Np63 RNA interference down-regulated Ngf expression in TKE2 cells. Our In vitro study unveiled that neurite outgrowth of the mouse primary trigeminal ganglion cells (TGCs) was inhibited as co-cultured with mouse corneal epithelial cells (TKE2) transfected by Shp2-, Mek1/2-, or ∆Np63-targeted siRNA but not by Akt1/2-targeted siRNA. These defects manifested in Shp2 K14ce-cko mice resemble the symptoms of human neurotrophic keratopathy. In addition, corneal epithelial debridement wound healing was delayed substantially in the mutant mice. More specifically, the corneal epithelium thickness and corneal sensitivity reduced dramatically in Shp2 K14ce-cko mice. Our data revealed that Shp2 K14ce-cko resulted in corneal denervation. Herein, we generated a compound transgenic mouse strain, K14rtTA tetO-Cre ( TC) Shp2 flox/flox, in which Shp2 was conditionally knocked out from the K14-positive cells including corneal epithelium ( Shp2 K14ce-cko) upon doxycycline (dox) administration. It remains unknown whether corneal epithelium is also reciprocally important for the corneal innervation. Our privacy policy and terms can be found here.Corneal nerve fibers serving sensory, reflex and neurotrophic functions sustains the corneal homeostasis and transparency to warrant the normal visual function. Your personal information will be kept securely and will not be Please email including your name, address and amount donated. To change or cancel your donation, to request a receipt for your cheque, or any other questions, Please use your initials and surname in the transactionĭescription, for example R H Smith. To donate by direct bank transfer, use the following details: Please include your postcode and email address if you would like us to claim Gift Aid on your donation. The Conversation Trust (UK) is a non-profit company limited by guarantee (number 08158264), and a registered charity in England and Wales (number 1151436) and in Scotland (number SC044707).
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